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MMP9 Antibody (Biotin)抗体,orb14

9759,biorbyt
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  • ¥8450
  • biorbyt已认证
  • orb149759
  • 英国
  • 2025年12月03日
  • FC, ICC, IF, IHC, IP, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      MMP9 Antibody (Biotin)抗体

    • 抗体英文名

      MMP9 Antibody (Biotin)

    • 靶点

      MMP9

    • 浓度

      1 mg/ml

    • 应用范围

      FC, ICC, IF, IHC, IP, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb149759

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Biotin

    • 克隆性

      Monoclonal

    • 亚型

      IgG2a

    • 免疫原

      Fusion protein amino acids 1-708 (full length) of rat MMP9

    • 规格

      100 ug

    产品描述:Mouse monoclonal to MMP9 (Biotin). MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis. Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development. One mechanism of MMP regulation is transcriptional. Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein..

    别名:MMP9, MMP-9, Matrix metalloproteinase 9, CLG4B, 82kDa matrix metalloproteinase-9, Collagenease type 4 beta, GELB, Macrophage gelatinase, MANDP2, Type V collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B

    免疫原:Fusion protein amino acids 1-708 (full length) of rat MMP9

    克隆性:L51/82 (Formerly sold as S51-82)

    分子量:82kDa

    应用稀释比例:WB (1:1000), ICC/IF (1:100)

    应用注释:1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_112317.1

    Entrez:81687

    UniProt ID:P50282

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone L51/82. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) MMP9 Antibody (D) Composite.

    产品细节图片2
    Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Mouse Anti-MMP9 Monoclonal Antibody, Clone L51/82. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).

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    图标文献和实验
    相关实验
    • The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods

      These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent

    • The Avidin-Biotin Complex(ABC) Method

      , between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin

    • Biotinylation of Antibody

      sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room

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