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VAChT Antibody (APC)抗体，orb1497

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  • ￥8580
  • biorbyt已认证
  • orb149707
  • 英国
  • 2025年12月31日
  • ICC, IF, IHC, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      VAChT Antibody (APC)抗体

    • 抗体英文名：

      VAChT Antibody (APC)

    • 靶点：

      VAChT

    • 浓度：

      1 mg/ml

    • 应用范围：

      ICC, IF, IHC, WB

    • 宿主：

      Mouse

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb149707

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      APC

    • 克隆性：

      Recombinant

    • 亚型：

      IgG1

    • 免疫原：

      Synthetic peptide amino acids 521-532 of human VAChT

    • 规格：

      100 ug

    产品描述：Mouse monoclonal to VAChT (APC). VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene..

    别名:Vesicular Acetylcholine Transporter, VAChT, SLC18A3, Slc18a3, Solute carrier family 18 member 3, Solute carrier family 18 (vesicular acetylcholine) member 3, Solute carrier family 18 (vesicular monoamine) member 3, Vesamicol-binding protein, MGC12716, rVAT, Unc-17

    免疫原：Synthetic peptide amino acids 521-532 of human VAChT

    克隆性:S6

    分子量:56kDa

    应用稀释比例：WB (1:1000), IHC (1:200), ICC/IF (1:100)

    应用注释：A dilution of 1:50-1:200 of SMC-393 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:95.46mM Phosphate, 2.48mM MES and 2mM EDTA

    纯化:Protein A Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_003046.2

    Entrez:6572

    UniProt ID:Q16572

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT Antibody (D) Composite.

    产品细节图片2
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VAChT Antibody. (D) Composite.

    产品细节图片3
    Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone N6/38. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:1000.

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