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文献和实验Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
for the quantitative analysis of protein?protein interactions using the flow cytometry protein interaction assay (FCPIA). This method is based upon immobilizing protein on a polystyrene bead, incubating these beads with a fluorescently labeled binding partner
Mag-Binds Plant DNA Kit Magnetic 96 Protocol
preset at 65-70°C 4. Equipment for disrupting plant tissue (MM300 Mixer Mill or Geno/Grinder 2000) 实验步骤 Tissue Disruption Manual disruption: To prepare samples, collect plant sample in a 30 ml mortar and freeze
v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
tubes (Beckman Coulter, Fullerton,CA; cat. #344058). 3. Beckman SW-28 swinging bucket rotor. 4. Pasteur pipettes. 5. 1× Tris-buffered saline (TBS): 50 mM Tris-HCl, pH 7.5, and 150mM NaCl. To prepare, dissolve 6.05 g Tris and 8.76 g NaCl
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