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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
has been incubated in the PAXgene Blood RNA Tube for a minimum of 2 h at room temperature, in order to achieve complete lysis.Preheat a water bath or heat block (for microcentrifuge tubes) to 55℃, during first spin.We spin at 3750 rpm in a standard table
96-well RNA In Situ Hybridization Protocol
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle (Chromosoma 98 (1989), p81), but adapted to allow the screening of 96 RNA probes on whole-mount Drosophila embryos at the same time.Jasprien
Kingfisher Flex 96 Plant DNA High Pure Protocol
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user 1. Centrifuge capable of at least 3,000-5,000 x g 2. Rotor adapter for 96
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