- ￥5148
- Biorbyt
- orb66674
- 英国
- 2026年08月06日
- WB
- Mouse
- Mouse
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- 文献和实验
- 技术资料
- 抗体名：
GR-1 (C) Antibody, Mouse Monoclonal 抗体
- 抗体英文名：
GR-1 (C) Antibody, Mouse Monoclonal
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb66674
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ul
描述: Mouse monoclonal to GR1.Granulocyte-differentiation antigen-1 (GR-1) is also known as Lymphocyte antigen 6G (Ly-6G), a GPI-anchored cell surface murine protein with an apparent molecular weight of 21-25 kDa. GR-1 is expressed on mature granulocytes in bone marrow and peripheral tissues.
别名: anti-GR1 antibody,anti-Lymphocyte antigen 6G antibody,anti-Ly6G antibody,anti-Ly6G.1 antibody
应用稀释比例: WB:1:1000-1:5000
免疫原: C-terminal region of mouse GR1
分子量: 12
防腐剂: 0.02% Thimerosal
纯度: Caprylic Acid-Ammonium Sulfate precipitation
研究领域: Epigenetics
UniProt ID: P35461
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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