Phospho-Tau (Thr181) Rabbit Polyclonal Antibody 抗体，orb1878，Biorbyt产品图

Phospho-Tau (Thr181) Rabbit Po

lyclonal Antibody 抗体，orb1878，Biorbyt
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  • ￥3159 - 5850
  • Biorbyt已认证
  • orb1878
  • 英国
  • 2026年07月22日
  • WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Phospho-Tau (Thr181) Rabbit Polyclonal Antibody 抗体

    • 抗体英文名：

      Phospho-Tau (Thr181) Rabbit Polyclonal Antibody

    • 靶点：

      MAPT

    • 浓度：

      1mg/ml

    • 应用范围：

      WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb1878

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      详询

    • 规格：

      50 ul/100 ul/200 ul

    规格：50 ul产品价格：￥3159.0
    规格：100 ul产品价格：￥4914.0
    规格：200 ul产品价格：￥5850.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Phospho-Tau (Thr181) Rabbit Polyclonal Antibody

    别名: tau; DDPAC; FTD1; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; Tau-PHF6; tau-40; Mtapt; PHF-tau; MAPT_0N4R; MAPT_1N4R; MAPT_2N4R; RNPTAU; pTau; TAU_BOVIN; MAPT; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); TAU_HUMAN; TAU_MOUSE; TAU_RAT; microtubule associated protein tau; G protein beta1/gamma2 subunit-interacting factor 1; microtubule-associated protein tau, isoform 4; protein phosphatase 1, regulatory subunit 103; Tau-derived paired helical filament hexapeptide; MAPT | Tau (phospho-T181); p-Tau; phospho-Tau; p-MAPT; MAPT | Tau (phospho-Thr181)

    应用稀释比例: WB=1:500-2000

    免疫原: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Thr181 PK(p-T)PP

    分子量: 52 kDa

    预测反应性: Bovine, Canine, Equine, Rabbit

    防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化方法: Affinity purified by Protein A

    研究领域: Neuroscience

    UniProt ID: P10636

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Blank control (blue line): MCF 7 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Tau (Thr181) antibody (orb1878), dilution: 3 µg/10^5 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

    产品细节图片2
    Fixative-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Tau (Thr181)) Polyclonal Antibody, Unconjugated (orb1878) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Tau (Thr181)) Polyclonal Antibody, Unconjugated (orb1878) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Lane 3: Cerebellum (Mouse) Lysate at 40 ug, Lane 4: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-phospho-Tau (Thr181) (orb1878) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-70 kD, Observed band size: 50 kD.

    产品细节图片5
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-Tau (Thr181) Polyclonal Antibody, Unconjugated (orb1878) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

    产品细节图片6
    Tissue/Cell: SH-SY5Y cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Tau (Thr181)) polyclonal Antibody, Unconjugated (orb1878) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

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