- ￥3159 - 5850
- Biorbyt
- orb100969
- 英国
- 2026年07月22日
- IF, IHC-Fr, IHC-P
- Rabbit
- Human, Rat
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- 文献和实验
- 技术资料
- 抗体名：
E1 Ubiquitin Activating Enzyme Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
E1 Ubiquitin Activating Enzyme Rabbit Polyclonal Antibody
- 靶点：
UBA1
- 浓度：
1mg/ml
- 应用范围：
IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb100969
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
详询
- 规格：
50 ul/100 ul/200 ul
| 规格： | 50 ul | 产品价格： | ￥3159.0 |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥4914.0 |
| 规格： | 200 ul | 产品价格： | ￥5850.0 |
产品子类型: Primary Antibody
描述: E1 Ubiquitin Activating Enzyme Rabbit Polyclonal Antibody
别名: A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS; Sbx; Ube-1; UBA1_HUMAN; UBA1; Protein A1S9; Ubiquitin-activating enzyme E1; 6.2.1.45; UBA1_MOUSE; Ubiquitin-activating enzyme E1 X; Ubiquitin-like modifier-activating enzyme 1 X; Ube1ax; UBA1_RAT; ubiquitin like modifier activating enzyme 1; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing); ubiquitin-like modifier activating enzyme 1; UBA1, ubiquitin-activating enzyme E1 homolog (yeast); POC20 centriolar protein homolog (Chlamydomonas)
应用稀释比例: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
免疫原: KLH conjugated synthetic peptide derived from human UBE1 (961-1058/1058aa)
分子量: 118 kDa
预测反应性: Mouse
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
研究领域: Protein Biochemistry
UniProt ID: P22314
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Purification of E1 and E1-Like Enzymes
Ubiquitin-activating enzyme is the archetype for a family of enzymes catalyzing the ATP-coupled activation of ubiquitin and other class 1 ubiquitin-like proteins required for their subsequent conjugation to cellular targets. The general
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
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