BAC libraries are essential elements in doing large-scale genome research such as genome sequencing, physical maps, and gene cloning. This technology was used in applications for genomic analysis that included large-scale physical mapping andgenomic sequencing although this technology was developed much later than Yeast Artificial Chromosome (YAC) and cosmid cloning technologies. This service should be attributed to the technical advantages of BAC cloning over YAC and cosmid cloning system summarized as follows:
• Bigger insert size (up to 300 kb) than cosmid and other plasmid system.
• Much more stable than yeast.
• Bacterial clones and libraries grow much faster than YAC.
• Easier in handling and gridding during DNA preparation.
The Sketch Map of Basic BAC Library Construction Steps:
Our BAC Library Construction Service as Follow:
Arrayed/non-arrayed BAC Library An arrayed or non-arrayed BAC library is the preferred choice for researchers wishing to screen using hybridization techniques. An arrayed library is also the only template that will do for whole genome sequencing or physical mapping.
Screened/Pooled BAC Library A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive.