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- 2026年01月27日
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- 服务名称：
de novo Sequencing
- 提供商：
北京安必奇生物科技有限公司
de novo sequencing, in which sequencing analysis is performed without the aid of a reference genome, is necessary when sequencing a novel species or novel DNA sequence. Combining the latest next generation sequencing technologies, you can obtain high quality results by sequencing different library types and subsequent state-of-the-art bioinformatics.
de novo Sequencing Features and Benefits:
Reliable genome assembly
Cost effective
High throughput data
Low background
Greater accuracy and broader dynamic range
de novo Sequencing Workflow:
de novo Sequencing Applications:
Sequencing of unknown genomes or when no reference sequence is available
Samples with expected large structural variation e.g. cancer cells
Microbial sequencing – experimental strains, genomes with high plasticity
CD Genomics de novo Sequencing Service includes:
The whole genome sequencing on human, animal, plant, microbial.
The genome assembly, function annotations, comparative genomics and molecular evolution analysis.
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文献和实验De Novo Sequencing Methods in Proteomics
The review describes methods of de novo sequencing of peptides by mass spectrometry. De novo methods utilize computational approaches to deduce the sequence or partial sequence of peptides directly from the experimental MS/MS spectra
Using the Velvet de novo Assembler for Short‐Read Sequencing Technologies
. Bioinformatics 25: 2078‐2079. Li, R., Zhu, H., and Wang, J. 2010. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 20:265‐272
RAD Paired-End Sequencing for Local De Novo Assembly and SNP Discovery in Non-model Organisms
of interest or move from SNPs identified by RAD to a high-throughput genotyping assay. Paired-end sequencing of RAD fragments can alleviate these problems by generating a set of paired sequences that can be locally assembled into high-quality contigs up to 1 kb in length
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