Nur77 Rabbit Polyclonal Antibo

dy 抗体，orb6550，Biorbyt

价格￥3159 - 5850

品牌Biorbyt
货号orb6550
产地英国
更新日期2026年07月22日

应用范围IF, IHC-Fr, IHC-P, WB
宿主Rabbit
适应物种Mouse, Rat

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抗体名：
Nur77 Rabbit Polyclonal Antibody 抗体
抗体英文名：
Nur77 Rabbit Polyclonal Antibody
靶点：
NR4A1
浓度：
1mg/ml
应用范围：
IF, IHC-Fr, IHC-P, WB
宿主：
Rabbit
适应物种：
Mouse, Rat
保质期：
12 months from date of receipt.
抗原来源：
详询
目录编号：
orb6550
级别：
科研
库存：
99
供应商：
Biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
保存条件：
详询
形态：
Liquid
亚型：
IgG
免疫原：
详询
规格：
50 ul/100 ul/200 ul

规格：	50 ul	产品价格：	￥3159.0
规格：	100 ul	产品价格：	￥4914.0
规格：	200 ul	产品价格：	￥5850.0

类别: Antibodies

产品子类型: Primary Antibody

描述: Nur77 Rabbit Polyclonal Antibody

别名: GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3; Gfrp; Hbr-1; Hbr1; NGFI-B; NUR77-1; NUR77-2; TIS1; NR4A1_HUMAN; NR4A1; Early response protein NAK1; Nuclear hormone receptor NUR/77 (Nur77); Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3; NAK1; NR4A1_MOUSE; Nuclear hormone receptor NUR/77; Nuclear protein N10; NR4A1_RAT; Nerve growth factor-induced protein I-B (NGFI-B); nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1

应用稀释比例: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

免疫原: KLH conjugated synthetic peptide derived from human Nur77/GFRP (351-450/598aa)

分子量: 70 kDa

预测反应性: Human

防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

纯化方法: Affinity purified by Protein A

RRID: AB_10953518

研究领域: Immunology & Inflammation

UniProt ID: P22736

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Nur77 antibody (orb6550), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Fixative-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Nur77) Polyclonal Antibody, Unconjugated (orb6550) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-Nur77 (orb6550) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kDa, Observed band size: 73 kDa.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Nur77 (orb6550) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kD, Observed band size: 66 kD.

Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Nur77 Polyclonal Antibody, Unconjugated (orb6550) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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