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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
of amplification, as the mean length of target DNA is decreased: for templates of 300bp or less, denaturation temperature may be reduced to as low as 88℃ for 50% （G+C） templates （Yap and McGee, 1991）， which means one may do as many as 40 cycles
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
, clearance mounting hole Newport SN100C-F 2 High Precision Knob Adjustment Screw, 12.7mm travel, 100TPI Newport
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
blue tetrazolium in 70% dimethylformamide, 30% H2O. Store at -200 C in a glass container covered with foil. Does not freeze at this temperature. Levamisole (100X): 50 mM in H20. Store at -200 C. PMS (100X): 2 mg/ml phenazine methosulfate
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