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- 文献和实验
- 技术资料
- 抗体名:
Goat anti-TAS1R2 / GPR71 Antibody 抗体
- 抗体英文名:
Goat anti-TAS1R2 / GPR71 Antibody
- 靶点:
TAS1R2 / GPR71
- 应用范围:
ELISA, WB
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb19715
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 免疫原:
详询
- 规格:
100 ug
描述: Goat polyclonal antibody to TAS1R2
别名: anti TAS1R2 antibody, anti GPR71 antibody, anti taste receptor, type 1, member 2 antibody, anti T1R2 antibody, anti TR2 antibody, anti G protein-coupled receptor 71 antibody
应用稀释比例: ELISA: 1:32000, WB: 1-3 μg/ml
应用说明: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in Human Pancreas lysates (calculated MW of 95.2kDa according to NP_689418.2). Recommended concentration: 1-3 μg/ml.
分子量: 95.2
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
蛋白序列: CSKRCQSGQKKKP
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
RRID: AB_10767499
研究领域: Cell Biology
Entrez 基因 ID: 80834
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

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文献和实验Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
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