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- 文献和实验
- 技术资料
- 英文名：
CFSE
- 库存：
10
- 供应商：
研卉生物
- 规格：
10mg
Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.
| 产品描述 | Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. |
| 靶点活性 | Others |
| 体外活性 | The binding of CFDA-SE fluorescent dye to cells is stable. It is not toxic and does not modify the viability of labeled cells. CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling. CFDA-SE persists in cells 24 h after labeling, allowing the long-term detection of labeled cells[1]. |
| 体内活性 | In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs[2]. |
| 细胞实验 | The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 µM, 3 µM, 4 µM, 5 µM, 10 µM and 20 µM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.(Only for Reference) Cell lines: human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375 |
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文献和实验CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
一、原理荧光染料CFSE， 也可称为CFDA SE（5，6- carboxyfluorescein diacetate，succinimidyl ester） 即羟基荧光素二醋酸盐琥珀酰亚胺脂，是一种可穿透细胞膜的荧光染料，具有与细胞特异性结合的琥珀酰亚胺脂基团和具有非酶促水解作用的羟基荧光素二醋酸盐基团，这使得CFSE成为一种良好的细胞标记物。当CFSE以含有两个乙酸基团和一个succinimidyl ester功能基团的形式存在时，不具有荧光性质，而具有细胞膜通透性，能够自由进入
Tracing Tolerance and Immunity In Vivo by CFSE-Labeling of Administered Cells
the fluorescent dye 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE) to evaluate CD8+ T-cell effector function in vivo by flow cytometry. In this assay, peptide-pulsed and control target cells are labeled to different levels with CFSE
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