- ￥748
- tsbiochem
- CN
- T6802
- 2025年07月08日
11 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 英文名：
CFSE
- 库存：
10
- 供应商：
研卉生物
- 规格：
10mg
CFSE
产品编号 T6802 CAS 150347-59-4
别名: 5(6)-CFDA N-succinmidyl ester, CFDA-SE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, Carboxyfluorescein succinimidyl ester, Carboxyfluorescein succinimidyl ester, CFDA-SE, 5(6)-CFDA N-succinmidyl ester 5(6)-Carboxyfluorescein diacetate succinimidyl ester
Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.
| 产品描述 | Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. |
| 靶点活性 | Others |
| 体外活性 | The binding of CFDA-SE fluorescent dye to cells is stable. It is not toxic and does not modify the viability of labeled cells. CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling. CFDA-SE persists in cells 24 h after labeling, allowing the long-term detection of labeled cells[1]. |
| 体内活性 | In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs[2]. |
| 细胞实验 | The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 µM, 3 µM, 4 µM, 5 µM, 10 µM and 20 µM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.(Only for Reference) Cell lines: human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
CFSE
￥748
