Mouse IL-7 ELISA Kit

Product name:

Mouse IL-7 ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma

Assay type:

Sandwich

Sensitivity:

3.6 pg/mL

Range:

15.6-1000 pg/mL

Reacted Species:

Mouse

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Serum	95%	83%-116%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	457.2	24.1	5.3
2	20	124.7	4.8	3.8
3	20	33.0	1.7	5.3

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	607.2	34.8	5.7
2	24	148.4	9.2	6.2
3	24	43.9	2.6	5.9

Product overview:

KE10060 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-7 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-7. The assay recognizes mouse IL-7. An antibody specific for mouse IL-7 has been pre-coated onto the microwells. The mouse IL-7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-7 is added to detect the captured mouse IL-7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm

Storage Instructions:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:

A630026I06Rik, hlb368, Il 7, Il7, IL-7, interleukin 7, Interleukin-7