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EZ DNA Methylation-Gold™ Kit
现货
上海研卉生物科技有限公司
50 Rxns
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished usi
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. .
Format | Spin Column |
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DNA Recovery | > 75% |
Processing Time | 3 hrs |
Equipment | Microcentrifuge |
Binding Capacity | 5 µg/prep |
Elution Volume | ≥ 10 µl |
Conversion Efficiency | > 99% |
Input | Plasmid, genomic, and endonuclease digested DNAs. |
DNA sequencing results after bisulfite treatment.DNA with methylated CmpG (at nucleotide position #5) was processed using the EZ DNA Methylation-Gold™ Kit. The recovered DNA was amplified by PCR and then sequenced directly. The methylated cytosine at position #5 remained intact while the unmethylated cytosines (i.e., positions #7, 9, 11, 14 and 15) were completely converted into uracil following bisulfite treatment and detected as thymine following PCR.
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