- ¥1662
- ZYMO RESEARCH
- D5005
- 美国
- 2025年12月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
EZ DNA Methylation-Gold™ Kit
- 库存：
现货
- 供应商：
上海研卉生物科技有限公司
- 规格：
50 Rxns
ZYMO RESEARCH品牌 D5005 EZ DNA Methylation-Gold™ Kit DNA甲基化试剂盒 50 Rxns.
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished usi
EZ DNA Methylation-Gold™ Kit
About EZ DNA Methylation-Gold™ Kit
The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. .
| Format | Spin Column |
|---|---|
| DNA Recovery | > 75% |
| Processing Time | 3 hrs |
| Equipment | Microcentrifuge |
| Binding Capacity | 5 µg/prep |
| Elution Volume | ≥ 10 µl |
| Conversion Efficiency | > 99% |
| Input | Plasmid, genomic, and endonuclease digested DNAs. |
|
DNA sequencing results after bisulfite treatment.DNA with methylated CmpG (at nucleotide position #5) was processed using the EZ DNA Methylation-Gold™ Kit. The recovered DNA was amplified by PCR and then sequenced directly. The methylated cytosine at position #5 remained intact while the unmethylated cytosines (i.e., positions #7, 9, 11, 14 and 15) were completely converted into uracil following bisulfite treatment and detected as thymine following PCR.
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相关热销产品：货号
| 名称 | 规格 | |
| D5001 | EZ DNA Methylation Kit™ | 50rxns |
| D5002 | EZ DNA Methylation Kit™ | 200rxns |
| D5003 | EZ-96 DNA Methylation Kit (shallow-well) | 2*96rxns |
| D5004 | EZ-96 DNA Methylation Kit (deep-well) | 2*96rxns |
| D5005 | EZ DNA Methylation-Gold Kit™ | 50rxns |
| D5006 | EZ DNA Methylation-Gold Kit™ | 200rxns |
| R1050 | Quick-RNA MicroPrep | 50preps |
| R1051 | Quick-RNA MicroPrep | 200preps |
| R2050 | Direct-zol™ RNA MiniPrep | 50preps |
| R2052 | Direct-zol™ RNA MiniPrep | 200preps |
| D4008 | Zymoclean Gel DNA Recovery Kit | 200preps |
| D4014 | DNA Clean&Concentrator-5 | 200preps |
| T2001 | Frozen-EZ Yeast Transformation II Kit™ | 120rxns |
| T3001 | Z-Competent™ E.coil Transformation Kit | 20ML |
| T3002 | Z-Competent™ E.coil Transformation Kit | 60ML |
| Y1001 | Alpha-Factor Mating Pheromone | 240ul |
| Y1002 | Yeast Protein Kit | 200preps |
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文献和实验DNA methylation is an epigenetic event that affects cell function by altering gene expression
代产品比，它有更加简单、快速、试剂全面的诸多优势，让您的操作更为方便 。 EZ ChIP™（catalog #:17-371）的特点与优势 更简单：过柱法的DNA纯化过程免去了phenol-chloroform提取的不方便性和不稳定性 更快速：新添的蛋白 酶抑制剂等试剂使盒中包括处理标本及实验操作的全部试剂，免去以前某些试剂需要临时准备的过程 更高更好的实验重复性：试剂盒中包括阳性、阴性对照抗体和相关PCR引物，帮助您评估整个实验
DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group, catalyzed by DNA methyltransferase (DNMT), to the 5-carbon of cytosine in a CpG dinucleotide
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