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Tyrosine Hydroxylase Rabbit Po

lyclonal Antibody抗体，orb11539，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb11539
  • 英国
  • 2025年12月03日
  • IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Tyrosine Hydroxylase Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      Tyrosine Hydroxylase Rabbit Polyclonal Antibody

    • 靶点：

      TH

    • 浓度：

      1mg/ml

    • 应用范围：

      IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb11539

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      参考产品说明书

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from mouse TH (101-165/528aa)

    • 规格：

      50 ul

    产品描述：Tyrosine Hydroxylase Rabbit Polyclonal Antibody

    别名:DYT14; DYT5b; ple; Protein Pale; c; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyk2; TY3H_HUMAN; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH.

    免疫原：KLH conjugated synthetic peptide derived from mouse TH (101-165/528aa)

    预测反应性:Bovine, Canine

    分子量:60 kDa

    应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P24529

    Note:For research use only.

    产品细节图片1
    paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (orb11539) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

    产品细节图片5
    Sample: adipocyte (mouse) Lysate at 5-10 ug, model A, model B, model C are from different mice, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1/2000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

    产品细节图片6
    Sample: PC-3 (Mouse) Lysate at 30 ug, Primary: Anti-Tyrosine Hydroxylase (orb11539) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

    产品细节图片7
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (orb11539) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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