Neuronal thread protein AD7c-NTP Rabbit Polyclonal Antibody 抗体，orb10039，Biorbyt产品图

Neuronal thread protein AD7c-N

TP Rabbit Polyclonal Antibody 抗体，orb10039，Biorbyt
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  • ￥3159 - 5850
  • Biorbyt已认证
  • orb10039
  • 英国
  • 2026年07月22日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Neuronal thread protein AD7c-NTP Rabbit Polyclonal Antibody 抗体

    • 抗体英文名：

      Neuronal thread protein AD7c-NTP Rabbit Polyclonal Antibody

    • 靶点：

      AF010144.1

    • 浓度：

      1mg/ml

    • 应用范围：

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb10039

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      详询

    • 规格：

      50 ul/100 ul/200 ul

    规格：50 ul产品价格：￥3159.0
    规格：100 ul产品价格：￥4914.0
    规格：200 ul产品价格：￥5850.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Neuronal thread protein AD7c-NTP Rabbit Polyclonal Antibody

    别名: neuronal thread protein AD7c-NTP; AD7c-NTP.; AD7C-NTP;

    应用稀释比例: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    免疫原: KLH conjugated synthetic peptide derived from human Neuronal thread protein AD7c-NTP (301-375/375aa)

    分子量: 43 kDa

    预测反应性: Mouse, Rat

    防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化方法: Affinity purified by Protein A

    RRID: AB_10746664

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Blank control: SHSY5Y. Primary Antibody (green line): Rabbit Anti-Neuronal thread protein AD7c-NTP antibody (orb10039), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Neuronal thread protein AD7c-NTP) Polyclonal Antibody, Unconjugated (orb10039) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-AD7c-NTP (orb10039) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.

    产品细节图片4
    Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-Neuronal thread protein AD7c-NTP (orb10039) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 43 kDa.

    产品细节图片5
    Tissue/cell: Mouse brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-AD7c-NTP Polyclonal Antibody, Unconjugated (orb10039) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    相关实验

    • Polyclonal Antibody Production

      , if desired this is best accomplished by Protein A Affinity Chromatography.   上一篇：make an Antibody against a Peptides   下一篇：Immunization Protocol for Monoclonal Antibody Production

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986

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