- ￥7488
- Biorbyt
- orb152870
- 英国
- 2026年07月24日
- DOT, ELISA, ICC, IF, IHC, IP, WB
- Rabbit
- Human
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- 文献和实验
- 技术资料
- 抗体名：
Amyloid Fibrils (OC) Antibody (Biotin) 抗体
- 抗体英文名：
Amyloid Fibrils (OC) Antibody (Biotin)
- 靶点：
Amyloid Fibrils (OC)
- 应用范围：
DOT, ELISA, ICC, IF, IHC, IP, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb152870
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Polyclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ul
描述: Rabbit polyclonal to Amyloid Fibrils antibody (Biotin)
别名: OC, Fibrils, Amyloid Oligomer aß, A11, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, APP, A4, AD, Amyloid precursor protein, Amyloid-β
应用稀释比例: WB (1:1000), IHC (1:100)
应用说明: A 1:1000 dilution was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
免疫原: Fibrils prepared from human amyloid beta 42 peptide
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
纯化方法: Protein A Purified
特异性: Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.
研究领域: Neuroscience
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
SANDWICH ELISA FOR DETECTING SECRETE
/ml （d) 64 ng/ml （e) 32 ng/ml （f) 16 ng/ml （g) 8 ng/ml （h) 4 ng/ml （i) 2 ng/ml B．Procedure 1. Diluate mAb R6.5 (or CL203) to 10 ug/ml in PBS. Add 50 ul diluted antibody to wells. Incubate 37oC for 1 h, or O/N at 4oC. 2. Wash 4X
. The intensity of the biotin-linked fluorescence is amplified by adding a layer of biotinylated goat anti-avidin antibody followed by another layer of fluorescein-avidin DCS. After washing off the excess stain, a fluorescence anti fade solution, e.g. p
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