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- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant SARS-CoV Spike/RBD Protein (RBD, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
SARS Coronavirus spike蛋白|Coronavirus spike protein|Coronavirus spike(SARS, C-His)(产品说明)
蛋白名称:Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建:A DNA sequence encoding the receptor binding domain (RBD) of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with a C-terminal polyhistidine tag.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Arg 306
蛋白分子量:The recombinant receptor binding domain (RBD) of SARS-CoV (isolate:WH20) spike comprises 233 amino acids and has a predicted molecular mass of 26.5 kDa. The apparent molecular mass of the protein is approximately 35.1 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:AAX16192.1
蛋白氨基酸序列:Arg306-Phe527
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Liu T, et al. Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates.Nature biomedical engineering, PubMed ID: 33122853
3, Guo K, et al. Rapid single-molecule detection of COVID-19 and MERS antigens via nanobody-functionalized organic electrochemical transistors.Nature biomedical engineering, PubMed ID: 34031558
4, Agarwal DK, et al. Highly sensitive and ultra-rapid antigen-based detection of SARS-CoV-2 using nanomechanical sensor platform.Biosensors & bioelectronics, PubMed ID: 34583103
5, Li Y, et al. Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.iScience, PubMed ID: 35607524
6, Hwu AT, et al. Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration.Lab on a chip, PubMed ID: 35737382
7, Koklu A, et al. Convection Driven Ultrarapid Protein Detection via Nanobody-Functionalized Organic Electrochemical Transistors.Advanced materials (Deerfield Beach, Fla.), PubMed ID: 35772173
8, Shim JE, et al. Single-Nanoparticle-Based Digital SERS Sensing Platform for the Accurate Quantitative Detection of SARS-CoV-2.ACS applied materials & interfaces, PubMed ID: 35951983
9, Kassardjian A, et al. Modular adjuvant-free pan-HLA-DR-immunotargeting subunit vaccine against SARS-CoV-2 elicits broad sarbecovirus-neutralizing antibody responses.Cell reports, PubMed ID: 37053069
10, Yu W, et al. Biosensor-based active ingredient recognition system for screening potential small molecular Severe acute respiratory syndrome coronavirus 2 entry blockers targeting the spike protein from Rugosa rose.Biomedical chromatography : BMC, PubMed ID: 39126351
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动物)的 ACE2 可以与 SARS-CoV-2 受体结合域(RBD)并促进 SARS-CoV-2 假病毒的转导。与 SARS-CoV-2 相比,SARS-CoV 似乎在选择受体方面有稍宽的范围。研究进一步以 3Å 分辨率解析了猫 ACE2(cACE2)与 SARS-CoV-2 RBD 的复合体的低温电子显微镜(cryo-EM)结构,揭示了与 hACE2 类似的结合模式与 SARS-CoV- 2 RBD。这些结果为寻找 SARS-CoV-2 的中间宿主提供了亮点,并强调了监控易感宿主以防止进一步爆发的必要
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