- ￥5820
- Sino Biological
- 北京
- 40150-V08B1
- 2026年01月26日
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- 保存条件：
-20℃ 至 -80℃
- 保质期：
12个月
- 英文名：
SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
SARS Coronavirus spike蛋白|Coronavirus spike protein|Coronavirus spike(SARS, C-His)（产品说明）
蛋白名称：Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建：A DNA sequence encoding the S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667) was expressed with a C-terminal polyhistidine tag.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2μg/mL (100μL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1),the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1) is 400-850 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 14
蛋白分子量：The recombinant S1 subunit of SARS-CoV (isolate:WH20) spike comprises 665 amino acids and has a predicted molecular mass of 74.4 kDa. The apparent molecular mass of the protein is approximately 85.8 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：AAX16192.1
蛋白氨基酸序列：Met1-Arg667
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Torrente-Rodríguez RM, et al. SARS-CoV-2 RapidPlex: A Graphene-based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring.Matter, PubMed ID: 33043291
3, Liu T, et al. Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates.Nature biomedical engineering, PubMed ID: 33122853
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6, Zamzami MA, et al. Carbon nanotube field-effect transistor (CNT-FET)-based biosensor for rapid detection of SARS-CoV-2 (COVID-19) surface spike protein S1.Bioelectrochemistry (Amsterdam, Netherlands), PubMed ID: 34715586
7, Ohtsuka J, et al. Non-propagative human parainfluenza virus type 2 nasal vaccine robustly protects the upper and lower airways against SARS-CoV-2.iScience, PubMed ID: 34805782
8, Cieslewicz B, et al. Preclinical Immunogenicity and Efficacy of a Multiple Antigen-Presenting System (MAPSTM) SARS-CoV-2 Vaccine.Vaccines, PubMed ID: 35891233
9, Karunakaran V, et al. A non-invasive ultrasensitive diagnostic approach for COVID-19 infection using salivary label-free SERS fingerprinting and artificial intelligence.Journal of photochemistry and photobiology. B, Biology, PubMed ID: 36049288
10, Marzi R, et al. Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape.iScience, PubMed ID: 36507220
11, Gary EN, et al. Adenosine deaminase augments SARS-CoV-2 specific cellular and humoral responses in aged mouse models of immunization and challenge.Frontiers in immunology, PubMed ID: 36999023
12, Muñoz-Basagoiti J, et al. Cyanovirin-N binds to select SARS-CoV-2 spike oligosaccharides outside of the receptor binding domain and blocks infection by SARS-CoV-2.Proceedings of the National Academy of Sciences of the United States of America, PubMed ID: 36853940
13, D'Arco A, et al. Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy.International journal of molecular sciences, PubMed ID: 37298500
14, Liu S, et al. Utilizing noncatalytic ACE2 protein mutant as a competitive inhibitor to treat SARS-CoV-2 infection.Frontiers in immunology, PubMed ID: 38646520
15, Zhang J, et al. Virus-like structures for combination antigen protein mRNA vaccination.Nature nanotechnology, PubMed ID: 38802667
16, Wang C, et al. P-selectin Facilitates SARS-CoV-2 Spike 1 Subunit Attachment to Vesicular Endothelium and Platelets.ACS infectious diseases, PubMed ID: 38912949
17, Brustoloni CJ, et al. Systematic Study of Various Functionalization Steps for Ultrasensitive Detection of SARS-CoV-2 with Direct Laser-Functionalized Au-LIG Electrochemical Sensors.ACS applied materials & interfaces, PubMed ID: 39231012
全球首次！分离的新冠病毒中和抗体，还可同时抗 SARS 病毒
抗原表位，为预防和治疗新型冠状病毒肺炎 COVID- 19 提供了可能。图片来源：bioRxiv通常，冠状病毒中和抗体主要靶向介导进入宿主细胞的病毒表面三聚体刺突糖蛋白（Spike Glycoproteins，S 蛋白）。S 蛋白具有介导细胞附着的两个功能亚基：负责受体识别的 S1 亚基（存在四个核心结构域 S1 A/B/C/D）以及促使病毒与细胞膜融合的 S2 亚基。强大的中和抗体通常会靶向 S1 中的受体相互作用位点，从而使受体相互作用失效。之前的研究表明，SARS-CoV- 2 和 SARS-CoV
乙型肝炎、和HBsAg阳性的患者血清中前S1蛋白，前S1抗原阴转愈早，AHB患者的疗程愈短，预后也愈好。说明前S1抗原及其抗体的检测是急性乙型肝炎的临床诊断，疗效观察和判数据预后的良好指标。 前S1蛋白的分子生物学特性 1．乙肝病毒（HBV）的基因结构 HBV为嗜肝DNA病毒，由一个不完全双链DNA组成，约3200个氨基酸。长链L含4个开放读码框架，为病毒蛋白的编码区：S区、C区、P区、X区。短链S相当于长链的50%-100%，其不固定端可被内原性DNA多聚酶延长，使病毒成为完整
模子里配制的5%聚丙烯酰胺凝胶-8 mol/l尿素体系以及微型蛋白凝胶装置中简易快速的执行。 即使使用单链探针，用S1核酸酶分析真核RNA结构还是会有假象。例如，DNA:RNA杂合双链中微小的不配对就会对S1核酸酶有抗性。相反地，富含rU:dA序列的完美配对的杂合双链区容易受到酶切。一条单链DNA分子如果同时和两条不同的RNA分子杂交，则可以不被核酸酶作用。最后一点，S1核酸酶不能有效地切割与RNA突出环相对的一段DNA。大多数这些问题可以用改变S1核酸酶浓度、采用不同酶切浓度、使用
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