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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见产品批次
- 英文名:
Mouse Embryonic Fibroblast (MEF) Conditioned Media (100 mL)
- 库存:
需确认
- 供应商:
嵘崴达
- CAS号:
见产品说明
- 保存条件:
见产品说明
- 规格:
100 mL
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| AR005 | Mouse Embryonic Fibroblast (MEF) Conditioned Media (100 mL) |
| AR006 | Peroxynitrite (5 mL) |
| AR008 | N21-MAX Media Supplement (50X) (10 mL) |
| AR009 | N-2 MAX Media Supplement (100X) (5 mL) |
| AR010 | N21-MAX Insulin Free Media Supplement (50X) (10 mL) |
| AR011 | StemXVivo Cardiomyocyte Maintenance Media Supplement (50X) (10 mL) |
| AR012 | N21-MAX Vitamin A Free Media Supplement (50X) (10 mL) |
| AR013 | ITS Media Supplement (100X) (5 mL) |
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文献和实验Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Media (Catalog # AR005 , AR007 ). The protocol below has been used with the BG01V line
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
β-mercaptoethanol, 1000X Gibco® Mouse Embryonic Fibroblasts (Irradiated) Basic Fibroblast Growth Factor (bFGF) GlutaMAX™-I (100X) Collagenase Type IV for enzymatic passaging or StemPro® EZPassage™ Tool for mechanical
Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells
embryonic fibroblast (MEF) cells have a limited growth capacity and on prolonged passaging spontaneously immortalize at a low frequency. In contrast to transformation of primary MEF cells that requires the presence of two cooperating oncogenes, immortalized
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