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- 2026年01月06日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 样本：
液体
- 标记物：
卵白
- 适应物种：
不限
- 应用：
科研单位
- 检测方法：
酶联免疫法
- 检测范围：
不限
- 库存：
可定制
- 供应商：
瓦兰生物
- 规格：
96T
德国seramun 卵白检测试剂盒
Seramun公司生产的疫苗质控试剂盒，以高精度、高质量和操作简便为特点，大量应用于流感、狂犬、脊灰等疫苗的生产质控。目前其产品已被葛兰素（GSK）、凯龙（Chiron）、ID Biomedicals、礼莱（Lily）、Wellstat Biologics Corporation USA等疫苗生产厂家作为指定质控产品。
Vaccine control
Highly sensitive ovalbumin ELISA kits detect traces of ovalbumin in vaccine samples based on viral pathogens cultured in chicken eggs. The sensitive Bovine serum albumin ELISA kit ist intended to characterize the purity of vaccine samples derived form bacterial or viral pathogens cultured in fetal calf serum containing medium.
| Product | Order No. | Amount | Download |
|---|---|---|---|
| Serazym® Ovalbumin (37 °C incubation, 7 standards) |
E-041a | 1x 96 wells |  Instruction for use Safety data sheet Flyer |
| Serazym® Ovalbumin (room temp. incubation, 6 standards) |
E-041c | 1x 96 wells | Instruction for use Safety data sheet Flyer |
| Serazym® Bovine Serum Albumin | E-048 | 1x 96 wells | Instruction for use Safety data sheet Flyer |
| Serazym® Bovine Serum Albumin (BSA) sensitive | E-108 | 1x 96 wells | Instruction for use Safety data sheet Flyer Flyer |
另我公司还提供美国Cygnus technologies, Inc.（赛纳斯科技公司）的检测试剂盒：Cygnus是美国一家专门从事生物工程和制药过程中残留物检测的一系列产品，包括大肠杆菌试剂盒,BSA试剂盒,蛋白A生物污染物，CHO宿主蛋白残留，Vero cell HCP ELISA试剂盒，酵母菌宿主蛋白残留检测试剂盒等都有广泛应用；
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples should be centrifugated adequately and no hemolysis or granule was allowed.
Materials required but not supplied
1. Standard microplate reader(450nm)
2. Precision pipettes and Disposable pipette tips.
3. 37 ℃ incubator
Precautions
1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
3. Mix all reagents before using.
Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)
Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml | 0.3ml |
| Sample diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure plate membrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
8、4、2、1、0.5、0 ng/ml.
Reagent preparation
20×wash solution:Dilute with Distilled or deionized water 1:20.
Assay procedure
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3. Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not
appear uniform, gently tap the plate to ensure thorough mixing.
8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results
- This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
- First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
- To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
- Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
- The sensitivity by this assay is 0.1 ng/ml.
- Standard curve
Storage： 2-8℃.
validity： six months.
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
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文献和实验一、反应曲线 首先，我们了解一下化学反应的时间与吸光度一个曲线图，可以划分为四个区：延迟区、等速区、过渡区和平衡区，如下图所示： 二、反应原理 对于延迟区来讲，无规律可寻，对于指标检测没有意义。 等速区对应的指标检测方法是速率法。速率法又称连续监测法，是在测定酶活性或用酶法测定代谢产物时，连续选取时间-吸光度曲线中线性期内4个以上测光点作为读数点，并以单位时间吸光度变化值计算结果。线性期就是此期间内各测光点之间的吸光度差值相等，此线性期对酶促反应的底物来说属于零级反应。其测光点一般设置在加入
亦称蛋白。作为鸟类卵三级卵膜的一种，充斥于卵黄膜外侧和卵壳膜内侧之间的透明溶胶状物质。它是卵从输卵管下降期间，由管壁分泌而附加在卵黄外的物质。
在鸟卵，随着孵化的进行，而卵白失去水分，粘性增加，重量显著减少。这种情况下，于包裹卵白的浆尿膜末端所产生的皱襞则称为卵白囊。
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