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文献和实验Cationic Liposome-Mediated Transfection with Lipofectin Reagent
Since their original description, liposomes have been discussed as vehicles that could be used as carriers of pharmaceutically active agents (1 ), and their potential for use as carriers of genetic information has been examined
Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from Panomics Modified protocol below Step 1: Make complexes Note: Do not scale up more than 3x Thaw siRNA and DeliverX reagent Dilute siRNA into buffer 1 10 uM siRNA 2.17 ul Buffer
siRNA transfection protocol (6 well plate)
75 μ l OMEM + 2.5 μ l SilentFect (BioRad). Mix gently by pipetting 3x. Sit RT 2-5 mins. 75 μ l OMEM + 1.1 μ l siRNA (20 μ M stock => 20 nM final). Mix gently by pippetting 3x. Combine siRNA and transfection solution
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