mericon DNA Bacteria Kit (100)

MicroElute DNA Clean-Up Kit

Clean-up Spin Protocol For DNA fragments (30bp-100 bp)    1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed

Isolation of genomic DNA from bacteria

DNA with 40 mL 5M NH4Ac and 250 mL isopropanol, incubate at RT for 5 min.   Centrifuge for 10 min., wash pellet twice with 70 EtOH, dry pellet, dissolve in 100 mL TE.   Determine the DNA concentration by measuring the absorbance

Plasmid DNA Isolation from Bacteria

with a heat-sealed end). 8. Wash DNA by dipping end of rod into 1ml of 70% ethanol for 30 sec. 9. Resuspend DNA in 100-200μl TE buffer. Complete resuspension may take several days. 10. Store DNA at 4EC short term, -20 or -80EC long term 11. After DNA