25mL Reservoir - Sterile

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

(as air vent) into one hole and the free ends of the hard and soft tubing through the other 2 holes in the cap into the 250 ml glass bottle (as reservoir) (Fig. 2). Ensure that the hard tubing reaches the bottom of the reservoir (as outflow tubing

Crystallization of Kinesin Family Motor Proteins

KCl, 1 mM EGTA, 1 mM DTT, 0.9 M Li2SO4Reservoir: 20 mM PIPES pH 7.5, 1.8 M Li2SO4 or15 mg/ml protein in 10 mM PIPES pH 7.5, 50 mM NaCl, 1 mM EGTA, 1 mM DTT, 0.5 mM NaN3, 0.9M (NH4)2SO4Reservoir: 1.8M (NH4)2SO4,50 mM NaClKozielski et al., 1997aSack et

Establishment of a Primary Culture

containing PBS. Allow the tissue pieces to settle.   Remove the PBS with a sterile pipette and add 25 ml of trypsin, a proteolytic enzyme. Stir the solution gently at 37° C for 15-20 minutes.   Allow the larger