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- 抗体英文名：
Phospho-AMPK-beta-1 (Ser108) Antibody
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Monoclonal Antibody Production Protocol
for 5 - 10 min. Aspirate the medium, resuspend pellet and wash again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free
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