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- 抗体英文名：
Phospho-Na,K-ATPase alpha-1 (Ser23) Antibody
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文献和实验to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q
1.4 M NaCl 81 g NaCl 40% isopropanol 400 ml isopropanol up to 1 liter with Q check to make sure pH is 3.0 Affigel Blue Running Buffer 10 mM K2HPO4 2.28 g K2HPO4 0.15 M NaCl 8.2 g NaCl 0.02% azide 0.2 g Na azide
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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