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- 文献和实验
- 技术资料
- 抗体英文名:
p35/25 (C64B10) Rabbit mAb
- 是否单克隆:
多克隆
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文献和实验fractions in 5 ml tubes with 700 ul 1 M Tris pH 9.0 already in bottom of tube to neutralize (collect at ~5 min/fraction).25 tubes are sufficient for collection. In general, you can see high Ab concentrations in 7-8 tubes.Test 1 ul on pH paper.Read OD to know
(collect at ~5 min/fraction). 25 tubes are sufficient for collection. In general, you can see high Ab concentrations in 7-8 tubes. Test 1 ul on pH paper. Read OD to know Ab concentrations. OD (Absorbance at 280 nm)/1.35 = X
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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