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3years
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Neobioscience
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-20°C
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文献和实验Solution and Solid-State NMR of Lipids
Lipid structure and dynamics are of first importance for cellular function. Lipids such as phosphatidyl inositol (PtdIns) are essential in signaling pathways, as they are recognition sites at the membrane surface. Their head-group or chain
PROCEDURE 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) 2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid solution and vortex again 3. Centrifuge sample at 10
solution and vortex again 3. Centrifuge sample at 10,000 xg for 2 minutes 4. Collect the bottom phase (contains lipids)
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