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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期:
见产品外包装
- 英文名:
RNA, MS2 from bacteriophage MS2
- 库存:
需确认
- 供应商:
嵘崴达
- CAS号:
见产品外包装
- 保存条件:
见产品外包装
- 规格:
500 μL (10 A260 units)
RNA, MS2
from bacteriophage MS2
General description
RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.
Application
RNA, MS2 has been used:
• as a standard to assess RNA quantification methods
• in RNA extraction
• in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
• for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
• for structural and functional studies.
• to stabilize RNA during cDNA synthesis
• to improve RNA yield during RNA extraction or isolation
Quality
典型分析:根据现在的质量控制步骤,不会有蛋白质和宿主核酸。
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| 10165948001 | RNA, MS2 from bacteriophage MS2 | 500 μL (10 A260 units) | |
| 11296744001 | Mycoplasma Detection Kit 支原体检测试剂盒 | 1 kit (25 tests) | |
| 10742988001 | Restr.-Endonucl. Dpn I, 1000u | 1,000 U (10 U/µl) |
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- 作者
- 内容
- 询问日期
文献和实验T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
of a variety of proteins, its inability to serve as an efficient substrate for bacteriophage RNA polymerases apparently restricted its actual potential as a photocrosslinking agent. In this chapter, in vitro transcription conditions that allow for template
(hnRNA)和小核RNA(snRNA)。hnRNA是mRNA的前体;snRNA参与hnRNA的剪接(一种加工过程)。自1965年酵母丙氨酸tRNA的碱基序列确定以后,RNA序列测定方法不断得到改进。目前除多种tRNA、5SrRNA、5.8SrRNA等较小的RNA外,尚有一些病毒RNA、mRNA及较大RNA的一级结构测定已完成,如噬菌体MS2RNA含3569个核苷酸。 大多数天然RNA分子是一条单链,其许多区域自身发生回折,如可以配对的碱基相遇(A与U,G与C
Introduction of Hypermodified Nucleotides in RNA
methylaminomethyl-2-thiouridine (mnm5 s2 U), methylcarboxymethyl-2-thiouridine (mcm5 s2 U), and 2-thiomethyl-N -6-carbamoylthreonyl-adenosine (ms2 t6 A). We also describe methods for using these nucleoside phosphoramidite reagents to synthesize RNA oligonucleotides
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