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- 2025年09月03日
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- 文献和实验
- 技术资料
- 保质期：
见产品外包装
- 英文名：
BM Blue POD Substrate, precipitating
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见说明书
- 保存条件：
2-8°C
- 规格：
100 mL
3,3′-5,5′-Tetramethylbenzidine (TMB)
一般描述
BM blue is a chromogenic substrate for Peroxidase (POD) designed for precipitating enzyme immunoassays. It develops a permanent, dark blue band or spot at the POD binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It is more sensitive than DAB and gives less background staining than Alkaline Phosphatase reactions.应用
BM Blue POD Substrate, precipitating, is a colorimetric peroxidase substrate and can be used in a variety of applications:- Western blot
- Northern blot
- Southern blot
产品规格
Formula: C16H20N2Color: dark blue
Solubility in water: insoluble
Evaluation: visually
外形
Solution, ready-to-use制备说明
Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.| 11004760001 | Random Primed DNA Labeling Kit DNA标记试剂盒 | 1 kit (50 labeling reactions) |
| 11207733910 | Anti-digoxigenin POD-conjugate | 150 U |
| 11442066001 | BM Blue POD Substrate, precipi | 100 ml |
| 11633716001 | Anti-Digoxigenin-POD (poly) | 50 U |
| 11585606910 | DIG High Prime 160ul | 160 µl (40 labeling reactions) |
| 11062590001 | DNA MW-Marker VI | 50 µg (1 A260 unit) |
| 11277073910 | DIG RNA Labeling Mixture, 10x | 40 µl (20 reactions) |
| 3353591910 | DIG Gel Shift Kit, 2nd generat凝胶转移试剂盒 | 1 kit |
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文献和实验，不能多次检测；在探针拨离前，需要使用二甲基甲酰胺先将膜上沉淀的颜色去除。 其他的底物和抗体 在整个DIG系统中，还有其他的一些底物和多种荧光抗体，例如Fast Red Tablets, BM Purple, HNPP Fluorescent Detection Set 和DAB POD Substrate ；供不同研究的需要。
Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA)
), which yield green, orange and blue colors, respectively. A table of commonly used enzyme-substrate combinations is included in Appendix D. The Sensitivity of the Sandwich ELISA is Dependent on Four Factors: The number
by mapping of the new positions for uncharacterized remodelers. It is also noteworthy that mononucleosomes do not recapitulate all chromatin properties (Hansen, 2002). Consequently, all conclusions derived from using this substrate may not always apply
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