- 询价
- roche
- 4743725001
- 2025年09月04日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见产品外包装
- 英文名：
Expand™ High FidelityPLUS PCR System, dNTPack
- 库存：
已停产
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 保存条件：
−20°C
- 规格：
125 UNITS
Expand™ High FidelityPLUS PCR System, dNTPack
General description
The Expand High FidelityPLUS PCR System is a blend of Taq DNA Polymerase and a thermostable proofreading protein that lacks polymerase activity.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High FidelityPLUS PCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High FidelityPLUS PCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.
Application
• The Expand™ High FidelityPLUS PCR System is designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This convenient dNTPack is the product of choice for the development of a new amplification assay or the optimization of an existing assay. PCR
• RT-PCR
• Primer extension
• Carryover prevention
It has been used in the ultradeep pyrosequencing methods, inverse PCR and to measure the repeat length of CGG sequences.
| 4743725001 | Expand HiFi PLUS PCR System dN | 125 U |
| 10109134001 | RNase, 500 mg 核糖核酸酶 | 500 mg |
| 10127833001 | 3-HBDH, Grade II | 10 mg (2 ml) |
| 11680293001 | X-gal, 100mg | 100 mg |
| 11888382001 | Titan One Tube RT-PCR System ( | 25 reactions |
| 10662046001 | b-Galactose Dehydrogenase S脱氢酶 | 50 U |
| 10745782001 | DNA, Lambda | 1 ml (5 A260 units) |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Expand High Fidelity PCR--高保真PCR
Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products
Expand High Fidelity PCR (Boehringer) Steve Hahn Last modified 12/12/99 Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20
（Expand High Fidelity, 3.5 U/μL）10×PCR DIG probe synthesis mix （2 mM dATP, dCTP, dGTP, 1.9 mM dTTP,及0.1 mM DIG-11-dUTP, pH 7.0）Expand high fidelity buffer with MgCl2 （10×）方法步骤：以下反应条件主要参照PCR DIG probe synthesis kit 制造厂商所提供的产品说明。1） 取一支0.5 mL微量离心管，依下表逐一加入各项
技术资料暂无技术资料 索取技术资料
