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文献和实验Desalting Oligonucleotides by Gel Filtration
Desalting Oligonucleotides by Gel Filtration General Desalting or buffer exchange on a gel filtration column is dependent on volume, not quantity of DNA in solution. Many sizes of gel filtration columns are available
E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
Buffer before use. 3. Add 250 ul absolute ethanol (96-100%, room temperature) to the sample and mix thoroughly by vortexing. 4. Apply sample from step 3 to HiBind® MicroElute RNA column inserted in a 2 ml collection tube (supplied
LABELING GC-GLOBULIN WITH FLUORESCEIN
Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer
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