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文献和实验化,只能说在在细胞凋亡途径中Akt参与,但不能说明Akt与细胞凋亡途径的上下游关系。 所有建议楼主使用Akt的inhibitor来证明最好。 以上是我个人一点想法,good luck! yuanyuan_626 rarazhuzhu wrote: 谈谈我的看法 楼主想要验证细胞凋亡途径中是否有Akt/PKB参与 首先要确认你的细胞在某种刺激
Nijmegen-Bethesda Assay to Measure Factor VIII Inhibitors
Hemophilia A is an inherited bleeding disorder caused by a deficiency of factor VIII coagulant activity (FVIII:C). Patients are treated with infusions of either plasma-derived or recombinant factor VIII. However, some patients develop
for the determination of mTOR inhibition by rapamycin and WYE-125132 in assays of recombinant mTOR enzyme, immunprecipitated native mTOR complexes, growth factor- and amino acid-induced cellular phosphorylation cascades as well as the PI3K/AKT/mTOR hyperactive breast
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