Kdo2-Lipid A (sodium salt)

Epithelial Sodium Channel in Planar Lipid Bilayers

has been implicated in several diseases, including salt-sensitive hypertension (Liddle’s syndrome), salt-wasting syndrome (pseudohypoaldosteronism type I), pulmonary edema, and cystic fibrosis. We have utilized planar lipid bilayers, a well-defined system that allows

Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ion

-related techniques. Currently available protocols for the preparation of GUVs work only at very low salt concentrations, thus precluding experimentation under physiological conditions. In addition, the GUVs thus obtained lack membrane compositional

Planar Lipid Bilayer Experiment平面脂双层实验

pores and 1-2V for large ones.8、Note on chart recorder paper: sample using (and amount used), salt solution and pH, gain, mV applied, chart speed, date, chart full scale.9、Fill the chamber with salt solution (after lipid coated has been dried