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Xanthine Oxidase Fluorometric

Assay Kit
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  • Cayman已认证
  • USA
  • 2025年07月16日
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      Neobioscience

    • 保存条件

      -20

    • 规格

      96 Well

    XO catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful ROS. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman's XO Assay provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength between 520-550 nm and an emission wavelength between 585-595 nm.

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    相关实验
    • Hydrogen Peroxide Assay for Amine Oxidase Activity

      Amine oxidase activity can be assessed by measuring the conversion of substrate to product, or by measuring the formation of concomitantly formed hydrogen peroxide. Fluorometry offers greater sensitivity and selectivity than conventional

    • Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes

      vesicles were used, and sterol oxidation was initiated either by the peroxy radical generator 2,2′-azobis (2-amidinopropane) dihydrochloride or by the enzyme cholesterol oxidase. The data gathered from this assay may be used to examine the effects of water

    • Methods for the Detection of D-Amino-Acid Oxidase

      al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog

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