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文献和实验Catalytic Assays for Human Cytochrome P450
Cytochrome P450 (P450) enzymes catalyze the blotransformatlon of a broad range of structurally diverse foreign chemicals and endogenous substances. Many P450 enzymes have been isolated and identified in liver and other tlssues, including
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
Evaluation of Cytochrome P450 Inhibition in Human Liver Microsomes
Evaluation of lead compounds for P450 inhibition in human liver microsomes has been widely accepted as an in vitro approach to assess drug interaction potential. This chapter describes a detailed traditional CYP inhibition protocol
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