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文献和实验Studies of the Ubiquitin Proteasome System
6.3 ) Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype 20× ubiquitylation buffer 2 for E3 assays (see recipe ) Mutant ubiquitins: lysine‐less (K0), K48R, K63R, K48 only, K63 only (BioMol
Reconstitution of CHIP E3 Ubiquitin Ligase Activity
to monitor the folding status of the CFTR protein, and we have successfully reconstituted this activity using a recombinant CFTR fragment consisting of the cytosolic NBD1 and R domains. We have found that efficient ubiquitination of substrates requires
【分享】Human press METHODS IN MOLECULAR BIOLOGY
. Protein Purification Protocols, 1st. Edited by S. Doonan. 1996 60. Protein NMR Techniques, 1st . Edited by D. G. Reid., 1996 61. Protein and Peptide Analysis by Mass Spectr- ometry, Edited by Chapman, John R., 1996 62. Recombinant Gene Expression
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