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- 文献和实验
- 技术资料
- 供应商:
Neobioscience
- 保存条件:
+4
- 规格:
1 x 100.0 x µg
It is recommended for use with the following products:
ALX-804-010 MAb to FasL (H11)
ALX-804-127 MAb to FLIP (Dave-2)
ALX-804-190 MAb to Laminin-2α (4H8-2)
ALX-804-202 MAb to E-Cadherin (ECCD-2)
ALX-804-258 MAb to α-Synuclein (15G7)
ALX-804-333 MAb to Smac/DIABLO (10G7)
ALX-804-366 MAb to Smac/DIABLO (9H10)
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文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Immunohistochemistry using Anti-Ganglioside Antibodies
')2 or FITC-conjugated goat anti-mouse IgG F (ab')2 diluted at 1:1000 each with 1% BSA in PBS for 1 hr. at room temperature. 7. Wash with 1% BSA in PBS 5 times. 8. Apply drops of fluorescence mounting medium on section and mount micro cover glass
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