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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protector RNase Inhibitor
- 保质期:
见产品外包装
- 供应商:
嵘崴达
- 保存条件:
−20°C
- 库存:
需确认
- 规格:
10000 units
Protector RNase抑制剂
Protector RNase Inhibitor
别名: RNase抑制剂, protector
General description
组分
Protector RNase抑制剂(40 U/μl)储存液:20 mM HEPES-KOH,50 mM KCl,8 mM 二硫苏糖醇, 50% 甘油(v/v)pH 7.6左右(+4°C)。
Protector RNase Inhibitor inactivates a wide spectrum of RNases, including
• RNase A
• RNase B
• RNase T2
Thus, Protector RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems. For instance, it can:
• protect mRNA during cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), or in vitro transcription/translation reactions
• protect viral RNA during in vitro virus replication
• inhibit RNases during RNA isolation and purification
• be used in RNase protection assays
• help prepare RNase-free antibodies
Note: Protector RNase Inhibitor does not interfere with enzymes commonly used to prepare or analyze RNA.
Specificity
Protector RNase抑制剂完全不会对RNase T1与RNase 1造成影响。
Protector RNase抑制剂(20 U/ 20 μl反应)能够抑制多达1 ng RNase A,160pg RNase B以及0.03 U/μl(反应体系)RNase T2的活性。
RNase H的活性不受Protector RNase抑制剂的抑制。
热灭活:> 65 °C
Features and Benefits
- 基于热稳定性的RNase抑制剂:即使使用热稳定性逆转录酶(如Transcriptor逆转录酶)的条件下,Protector RNase抑制剂仍保持稳定。
- 兼容广泛的反应条件:Protector RNase抑制剂在pH 5.0至9.0条件下和+25°C至+55°C的温度下都具有活性(+60°C时仍保留部分活性)。
- 避免各种RNA分析应用中的干扰:即使升高到标准浓度的16倍仍保持正常效能。
- 持续提供高纯度产物:每一批次的产品都进行严格质控流程,经实时定量RT-PCR等标准技术检测,确保不含核酸内切酶、核糖核酸酶或切割活性。
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文献和实验The 25A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI)
Interferons (IFN) belong to a multigenic family of cytokines that activate the transcription of several genes through a signaling pathway involving cell-surface receptors (IFNAR), Janus kinases (JAK), and signal-transducing proteins (STAT
, would RNase out RNase inhibitor influence the function of RNase H, if it was added even before first stand synthesis? Answer 1. The main difference between all RNases are where they cleave the RNA (what site they recognize) and whether it is single
Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
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