MITOSOX RED MITOCHONDRIAL SUPE

Analysis of Mitochondrial DNA Mutations

Human mitochondrial DNA (mtDNA) is a closed circular genome of 16,569 bp (1 ), encoding 13 subunits of four enzyme complexes (Complexes I, III, IV, and V) in the oxidative phosphorylation system (2 ). Mutations of mtDNA

Fluorescence-Activated Cell Sorting Analysis of Mitochondrial Content, Membrane Potential, and Matrix Oxidant Burden in Human Lymphoblastoid Cell Line

(TMRE) to assess mitochondrial membrane potential, and MitoSOX Red to assess mitochondrial matrix oxidant burden. Representative results of FACS-based mitochondrial analyses demonstrate the variability of these three basic mitochondrial parameters

MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol

of DiIC1(5) are 638 nm and 658 nm, respectively. Cells labeled with DiIC1(5) can be analyzed by flow cytometry using 633 nm excitation and far red emission, and by fluorescence microscopy using standard filters for Alexa Fluor® 633 dye. 实验