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- invitrogen
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- 2025年07月11日
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货号：K497500
品牌：invitrogen
规格：195 UG X 3
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文献和实验Cosmid Cloning: Cell preparation, DNA packaging, and Cell Transfection
Cosmid Cloning: Cell preparation, DNA packaging, and Cell Transfection Protocol taken from Stratagene's Gigapack packaging extracts instruction manual Host bacteria (E. coli LE392)preparation: Pick one colony from fresh
Inducible shRNA Lentiviral Vectors
of your expression clone. 3) Cotransfect the ViraPower™ Packaging Mix and pLenti6/V5-GW/miR expression plasmid DNA into 293FT Cells using Lipofectamine™ 2000. 4) Harvest virus-containing supernatants 48-72 hours post-transfection. 4. Titering
【原创】lentivirus calcium phosphate transfection protocl
calcium phosphate transfection protocl： 1. Split 293FT cells about 30% confluence, overnight. 2. Thaw 2*HBS room temperature (not in 37 C). 3. For 10cm dish: 1.5ml tube, 4ug packaging plasmid A, 4ug packaging plasmid B, 4ug expression
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