- ￥100 - 9999
- invitrogen
- 15640055
- 2025年07月10日
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货号：15640055
品牌：invitrogen
规格：100ML- PLASTIC- E-Z HOLD
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文献和实验Method: Removal of Yeast Contamination from Lymphoblast Cultures
only antibiotic and not the antimycotic. Solutions: Wash media: (1 liter) To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 10.0 ml 2.5 M (100X) Hepes buffer 1.2 ml 50 mg/ml gentamicin reagent Filter sterilize
Cell Culture Media and Solutions
165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize through a 0.22 � cellulose acetate filter and store
(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
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