SOLiD Long Mate-Pair Lib Prep

Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology

Supported membranes, formed as a single continuous lipid bilayer on a solid substrate, such as silica, have been used extensively as a model for protein?protein and cell?cell interaction, to study the molecular interactions at interfaces

Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR

. First, the appropriate building blocks, either 5?iodo?substituted pyrimidine deoxy? or ribo?nucleoside phosphoramidites are prepared and incorporated by solid?phase synthesis. Following this, the protected oligonucleotides are ?on column? reacted with the acetylenic

Stripping Western Blots

, put in an eppendorf, rock 10-20’ at 4C and then spin 10’. Save supernatent―determine protein concentration 4. Immunoprecipitate 100 μg of protein (if you use K12 antibody, and 200 μg of protein if you use AB-3 antibody) in a total of 800 μl. Use 1 μg