FG,RUNNING BUFFER WITH EDTA (1

10X TBE BUFFER

  10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer

8.3 with acetic acid at room temp.Adjust volume to 500 ml.Running buffer is 1X TGOE buffer[Note that there is no Mg or EDTA in both the gel and in the running buffer.]

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer

% ammonium persulfate 30 microliters TEMED     pH 8.3 with acetic acid at room temp. Adjust volume to 500 ml. Running buffer is 1X TGOE buffer [Note that there is no Mg or EDTA in both the gel and in the running buffer.]