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- 2025年07月15日
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货号：4463877
产品名称：RACK CHANGER RACK SET,1.5 ML VIAL 12 PC
品牌：abi
关键字：abi，Applied Biosystems公司，4463877，RACK CHANGER RACK SET,1.5 ML VIAL 12 PC
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
(37℃) per 100mm; aspirate, tilting plates to loosen ppt; wash 2x more with 4ml PBS/plate.Add 10ml fresh PC12 Culture Media; return to 10% CO2 incubator.Repeat for each set of 4 plates.Incubate 36-48 hours, analyze.Reagents:PolylysineSigma5 mg
Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
3. Add 1.5 ml freezing solution, mix well, and aliquot 1 ml each into 3 sterile freezing vials (Nunc CryoTubes #363401). 4. Freeze slowly to -80deg.. This is accomplished by placing the vials in a styrofoam rack (the kind that 15 ml disposable sterile
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