FILTER,MIST FILTER

Filter Hybridization

ensures a low background level of nonspecific probe adherence to the membrane and, second, an extended period of filter prehybridization is not required. The inclusion of a large amount of SDS does, however, necessitate that the nucleic acids

Filter-Binding Assays

binds to a specific DNA sequence, passage through the filter will result in retention of a fraction of the protein-DNA complex by virtue of the protein component of the complex. The amount of DNA retained can be determined by using radioactively labeled

Filter Binding Assay for EBNA-1

Wash one 0.45um Millipore nitrocellulose filter (Cat# HAWP01300) in 1ml 0.4M KOH for 1 min; wash in 3ml dH2 O for >1min. Assemble filter apparatus: white plastic bottom support/funnel, nitrocellulose filter, white plastic top with syringe adapter