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- 4459353
- 2026年06月21日
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货号：4459353
产品名称：COVER KIT,G4212 5065-9985
品牌：abi
关键字：abi，Applied Biosystems公司，4459353，COVER KIT,G4212 5065-9985
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文献和实验Biotin labeling and hybridization of probe to chromosomes
Biotin labeling and hybridization of probe to chromosomes Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Biotin labeling of plasmid or genomic DNA. Remove slides
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
at –80°C. 3. Crush liver sample on dry ice and store in a prechilled 5 ml culture tube. 4. Add about 100 μg tissue (enough to almost cover round portion of a 5 ml culture tube) to a new chilled tube. 5. Add 1 ml PBS with 10 μl protease
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