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- WC016163
- 2025年07月14日
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货号：WC016163
产品名称：PERKIN ELMER Series 200 Autosmpl Com Kit
品牌：abi
关键字：abi，Applied Biosystems公司，WC016163，PERKIN ELMER Series 200 Autosmpl Com Kit
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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
Inverse PCR & Cycle Sequencing
solution (P/N 50611), and the Perkin Elmer ABI BigDye Terminator Cycle Sequencing Ready Reaction Kit (P/N 4303154). The use of other thermal cyclers and sequencing strategies will require different cycling parameters and sequencing protocols.
Natural History Museum Protocol for EST sequencing from lamb
: 4µl of x10 PCR buffer (see below) 4.8µl of dNTP mix (see below) 0.25 µl of Perkin Elmer Amplitaq Taq polymerase (equivalent to 1.25 units) 1µl of each primer (see below) 2µl of phage suspension from step (2) 27µl of water overlay
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