- 询价
- Biotium
- 美国
- 70031
- 2025年09月07日
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- 详细信息
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- 技术资料
- 保存条件：
4°C避光
- 英文名：
NERVE TERMINAL STAINING KIT II (COMPONEN 70031
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Nerve Terminal Staining Kits contain Biotium’s nerve terminal dyes and background reducing agents for washing away or quenching extracellular dye fluorescence. Kits are available with SynaptoGreen™ C4 (equivalent to FM®1-43), the fixable green nerve terminal dye AM1-43, or SynaptoRed™ C2 (equivalent to FM®4-64). ADVASEP-7 is a sulfonated Œ≤-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (equivalent to FM®1-43) to stain brain slices. SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps. Sulforhodamine 101 has been used as a quencher dye to reduce SynaptoGreen™ C4 fluorescence.
See the†Neuronal Probes Flyer for detailed information.
Nerve Terminal Dyes and Kits
| Product | Catalog No. | Features |
|---|---|---|
| SynaptGreen™ Dyes (Ex/Em ~480/660 nm in membranes) |
70020... 70053 | View full list of SynaptoGreen™ Dyes |
| SynaptoRed Dyes™ (Ex/Em ~510/750 nm in membranes) |
70021... 70050 | View full list of SynaptoRed™ Dyes |
| Background Reducers | ||
| ADVASEP-7 | 70029 | Sulfonated cyclodextrin that aids in removal of free dye during washes |
| SCAS | 70037 | Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7 |
| Sulforhodamine 101 | 80101 | Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes |
| Nerve Terminal Staining Kits | ||
| Nerve Terminal Staining Kit I | 70030 | Includes SynaptoGreen™ C4 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (A) | 70031 | Includes AM1-43 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (B) | 70031-1 | Includes AM1-43 and SCAS |
| Nerve Terminal Staining Kit III | 70032 | Includes SynaptoGreen™ C4 and Sulforhodamine 101 |
| Nerve Terminal Staining Kit V | 70034 | Includes SynaptoRed™ C2 and ADVASEP-7 |
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
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Cloning of Taq polymerase-amplified PCR products
" vector) Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3´ ends of PCR products. The linearized vector supplied in this kit has single, overhanging 3´ deoxythymidine (T) residues
